Journal: PLoS ONE

Article Title: Calcium Channel-Dependent Molecular Maturation of Photoreceptor Synapses

doi: 10.1371/journal.pone.0063853

Figure Lengend Snippet: Antibodies.

Article Snippet: PSD-95 , Mouse , 1∶200 , Purified recombinant rat PSD-95. , , ABR–Affinity bioReagents,Golden, CO, USA..

Techniques: Transduction, Recombinant, Purification, Sequencing

Journal: PLoS ONE

Article Title: Calcium Channel-Dependent Molecular Maturation of Photoreceptor Synapses

doi: 10.1371/journal.pone.0063853

Figure Lengend Snippet: ( A–L ) Vertical sections from P13-WT ( A, E and I ), P13-KO ( B, F, and J ), P30-KO ( C, G and K ) and P30-WT ( D, H and L ). The immunoreactivity of Veli3 is presented alone in the top row in grayscale ( A–D ). In the second row Veli3 (magenta) and glycogen phosphorylase (Glypho, green) signals are presented merged ( E–H ). PSD-95 immunoreactivity is shown in panels ( I–L ). Scale bar = 5 µm.

Article Snippet: PSD-95 , Mouse , 1∶200 , Purified recombinant rat PSD-95. , , ABR–Affinity bioReagents,Golden, CO, USA..

Techniques:

Journal: PLoS ONE

Article Title: Calcium Channel-Dependent Molecular Maturation of Photoreceptor Synapses

doi: 10.1371/journal.pone.0063853

Figure Lengend Snippet: Several protein–protein interactions are affected such as the association of Ribeye, Piccolo and Bassoon. The investigated proteins in the model are depicted with a red outline. The proteins with an abnormal expression and/or distribution are shown in grey. Synaptic ribbons form a complex with several other proteins such as CAST, RIM2, Rab3a, and Munc13. In the Ca V 1.4 (α1F) -KO, RIM2 expression is compromised. The vesicle related machinery (VAMP2, synaptophysin and complexin 3 and 4) is not affected in the Ca V 1.4 (α1F) -KO. Other presynaptic proteins such PMCA, PSD-95 and Veli3 are also affected in the Ca V 1.4 (α1F) -KO. Furthermore, β-Dystroglycan, a protein necessary for the invagination of bipolar cells is absent from the photoreceptor synapses in the Ca V 1.4 (α1F) -KO.

Article Snippet: PSD-95 , Mouse , 1∶200 , Purified recombinant rat PSD-95. , , ABR–Affinity bioReagents,Golden, CO, USA..

Techniques: Expressing

Journal: The Journal of comparative neurology

Article Title: Expression of Protocadherin-γC4 protein in the rat brain

doi: 10.1002/cne.24783

Figure Lengend Snippet: Primary Antibodies

Article Snippet: The GP anti-rat vesicular glutamate transporter 1 (VGLUT1) (catalog #AB5905; lot# 24080852; RRID: AB_2301751), the Ms mAb to rat PSD-95 (clone 6G6–1C9; catalog #MAB1596, Lot# LV1453199; RRID: AB_2092365) and the Ms mAb to actin (clone C4; catalog # MAB1501; lot# LV1547855; RRID: AB_2223041) were from EMD Millipore (Billerica, MA).

Techniques: Affinity Purification, Purification, Recombinant

Journal: The Journal of comparative neurology

Article Title: Expression of Protocadherin-γC4 protein in the rat brain

doi: 10.1002/cne.24783

Figure Lengend Snippet: Rat hippocampal cultures. (a-d) Double-label immunofluorescence with Rb anti-Pcdh-γC4 (γC4, green) and sheep anti-GAD (blue). Arrowheads show Pcdh-γC4 puncta at contact points between an incoming axon and dendrites. Filled arrowheads show Pcdh-γC4 puncta associated with GAD+ boutons. Empty arrowheads show Pcdh-γC4 puncta associated with axon-neuron contacts but not boutons. Panels a and b show the overlays. Note than in a and c the GABAergic axon (blue) contacts a dendrite of a GABAergic interneuron (blue, right side of the panel) and a pyramidal neuron (left side of the panel). Panels b and d show an axon (blue) contacting a dendrite of a GABAergic interneuron (blue). Note that Pcdh-γC4 puncta associated or not associated with the axon are larger in the contacts with interneurons in a and b, than in the contacts with the pyramidal neuron in a. (e-g) Triple label immunofluorescence with Rb anti-Pcdh-γC4 (green), sheep anti-GAD (blue) and Ms anti-gephyrin (magenta). Some Pcdh-γC4 puncta are localized at contact points between the axon and neuron (arrowheads), however, the majority of these Pcdh-γC4 puncta are adjacent to, but seldom co-localize with (arrow), gephyrin clusters. (h-j) Triple label immunofluorescence with Rb anti-Pcdh-γC4 (green), GP anti-VGLUT1 (blue) and Ms anti- PSD-95 mAb (magenta). Some Pcdh-γC4 puncta are associated with glutamatergic synapses (arrowheads) but the majority of Pcdh-γC4 puncta are adjacent to, but do not co-localize with VGLUT1 puncta or PSD-95 clusters. Scale bar = 10 μm.

Article Snippet: The GP anti-rat vesicular glutamate transporter 1 (VGLUT1) (catalog #AB5905; lot# 24080852; RRID: AB_2301751), the Ms mAb to rat PSD-95 (clone 6G6–1C9; catalog #MAB1596, Lot# LV1453199; RRID: AB_2092365) and the Ms mAb to actin (clone C4; catalog # MAB1501; lot# LV1547855; RRID: AB_2223041) were from EMD Millipore (Billerica, MA).

Techniques: Immunofluorescence

Journal: The Journal of comparative neurology

Article Title: Expression of Protocadherin-γC4 protein in the rat brain

doi: 10.1002/cne.24783

Figure Lengend Snippet: (a, c and e) glomerular layer of olfactory bulb. (b and d) granule cell layer of olfactory bulb. (f) External plexiform layer of olfactory bulb. (g and h) cerebellum. All panels show immunofluorescence with Rb anti-Pcdh-γC4 (magenta) and GP anti-VGLUT1 (blue). Panels a, b and f also show Ms PSD-95 immunofluorescence (green). Boxed areas in a, b and c are shown at higher magnification in c, d and e respectively. Panel a is mostly occupied by an olfactory glomerulus labeled OG in the center. The asterisk in a and c are placed in the same position inside the OG to serve as a reference. The arrows in a and c point to the same VGLUT1 puncta inside the glomerulus, which are also associated with Pcdh-γC4 puncta. Arrowheads in e point to Pcdh-γC4 puncta associated with VGLUT1 terminals. Numbers in b and d indicate some granule cell aggregates in the olfactory granule layer. (g) In the cerebellum, the arrow indicates the region with the highest concentration of bright Pcdh-γC4 puncta, which is at the boundary between Purkinje cell layer (PK) and granule cell layer (GR). Arrowheads point to a row of Pcdh-γC4 puncta in the molecular layer (ML). (h) Granule cell layer of the cerebellum. Asterisks indicate rows of granule cells. Note the absence of Pcdh-γC4 puncta in-between granule cells of the same row and the presence of Pcdh-γC4 puncta (arrowheads) separating VGLUT1 labeled synaptic glomeruli (blue) and granule cells. Scale bar represents 20 μm for a and b; 10 μm for c and d; 5 μm for e and f; and 12 μm for g and h.

Article Snippet: The GP anti-rat vesicular glutamate transporter 1 (VGLUT1) (catalog #AB5905; lot# 24080852; RRID: AB_2301751), the Ms mAb to rat PSD-95 (clone 6G6–1C9; catalog #MAB1596, Lot# LV1453199; RRID: AB_2092365) and the Ms mAb to actin (clone C4; catalog # MAB1501; lot# LV1547855; RRID: AB_2223041) were from EMD Millipore (Billerica, MA).

Techniques: Immunofluorescence, Labeling, Concentration Assay

Journal: The Journal of comparative neurology

Article Title: Expression of Protocadherin-γC4 protein in the rat brain

doi: 10.1002/cne.24783

Figure Lengend Snippet: (a-d) Double-label immunofluorescence with Ms anti- PSD-95 (magenta) and GP anti-VGLUT1 (blue) in cultured hippocampal neurons from WT mouse (a and c) and TCKO mouse (b and d). Boxed areas in a and b are shown at higher magnification in c and d. Scale bar = 20 μm for a and b and 10 μm for c and d.

Article Snippet: The GP anti-rat vesicular glutamate transporter 1 (VGLUT1) (catalog #AB5905; lot# 24080852; RRID: AB_2301751), the Ms mAb to rat PSD-95 (clone 6G6–1C9; catalog #MAB1596, Lot# LV1453199; RRID: AB_2092365) and the Ms mAb to actin (clone C4; catalog # MAB1501; lot# LV1547855; RRID: AB_2223041) were from EMD Millipore (Billerica, MA).

Techniques: Immunofluorescence, Cell Culture